Accepted articles

Instruments and methods of investigation

Three-dimensional fluorescence nanoscopy of single quantum emitters with highly efficient spatial phase modification of the point spread function based on spiral light beams

 a, b, c,  d,  d,  b,  d,  a, b, c
a Institute of Spectroscopy, Russian Academy of Sciences, ul. Fizicheskaya 5, Troitsk, Moscow, 108840, Russian Federation
b Moscow Institute of Physics and Technology (National Research University), Institutskii per. 9, Dolgoprudny, Moscow Region, 141701, Russian Federation
c Moscow State Pedagogical University, M. Pirogovskay, 1, Moscow, 119435, Russian Federation
d Samara Branch of the P.N. Lebedev Physics Institute, Russian Academy of Sciences, Novo-Sadovaya str. 221, Samara, 443011, Russian Federation

Far-field superresolution fluorescence microscopy (nanoscopy), awarded the Nobel Prize in Chemistry in 2014, has become one of the most powerful tools in multidisciplinary photonics applications. In this paper, we discuss the technique of three-dimensional nanoscopy with the detection of transformed fluorescent images of single quantum emitters (on the example of semiconductor colloidal quantum dots, QDs). Nanoscale spatial resolution at the reconstruction of all three coordinates of single QDs is achieved by the instrumental modification of the point spread function using highly efficient light phase spatial modulator (diffractive optical elements, DOEs). The DOE phase distributions, which ensure the formation of two-lobes light fields (with rotation of the intensity distribution during light propagation), were obtained on the basis of the optics of spiral light beams. The question of calculating DOEs providing the best conversion efficiency of light beams is discussed. The theoretical and experimental analysis of the accuracy of the method was carried out depending on the experimental parameters: the QD photoluminescence intensity, the signal exposure time, the laser excitation power, and the instrumental function of the microscope objective. It is shown that, for the studied CdSeS / ZnS QDs, the accuracy of determining the coordinates can reach values of a few nanometers at exposure times of ~ 100 ms.

Keywords: luminescence, microscopy, nanoscopy, diffraction limit, spatial resolution, single molecule, quantum dot, point spread function, adaptive optics, diffractive optical element, Lagger-Gauss modes, spiral beams, DHPSF, quantum optics, nanodiagnostics, sensorics
PACS: 42.79.−e, 78.55.−m, 78.67.Hc (all)
DOI: 10.3367/UFNe.2021.05.038982
Citation: Eremchev I Yu, Prokopova D V, Losevskii N N, Mynzhasarov I T, Kotova S P, Naumov A V "Three-dimensional fluorescence nanoscopy of single quantum emitters with highly efficient spatial phase modification of the point spread function based on spiral light beams" Phys. Usp., accepted

Received: 10th, March 2021, 3rd, May 2021

Оригинал: Ерёмчев И Ю, Прокопова Д В, Лосевский Н Н, Мынжасаров И Т, Котова С П, Наумов А В «Трехмерная флуоресцентная наноскопия одиночных квантовых излучателей с высокоэффективными фазовыми пространственными преобразователями функции рассеяния точки на основе спиральных пучков света» УФН, принята к публикации; DOI: 10.3367/UFNr.2021.05.038982

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