Accepted articles
On the 55th anniversary of the Institute of Spectroscopy of the Russian Academy of Sciences

Optical methods for the detection of single biomolecules: visualization, detection and sequencing of DNA molecules

P.N. Melentiev^a, A.S. Kalmykov^a, A.S. Gritchenko^a, M.P. Shemeteva^a, A.M. Safonova^a, M.S. Markov^a, V.I. Balykin^a, A.S. Bukatin^b, N.V. Vaulin^b, D.A. Belov^b, A.A. Evstrapov^b, D.A. Baklykov^c, A.V. Andriyash^c, A.A. Barbasheva^d, A.K. Kuguk^d, V.V. Ryzhkov^d, I.A. Rodionov^d, D.S. Kudryavtsev^e, V.A. Mozhaeva^e, L.V. Son^e, V.I. Tsetlin^e, B.N. Khlebtsov^f, M.S. Kobzev^g, Yu.O. Kuznetsova^g, B.T. Sharipov^g, A.S. Yashkin^g, Ya.I. Alekseev^b, g
^aInstitute of Spectroscopy, Russian Academy of Sciences, ul. Fizicheskaya 5, Troitsk, Moscow, 108840, Russian Federation
^bInstitute of Analytical Instrumentation, Russian Academy of Sciences, Petrodvorets, St. Petersburg, Russian Federation
^cDukhov Research Institute of Automatics, ul. Sushchevskaya 22, Moscow, 119017, Russian Federation
^dBauman Moscow State Technical University, ul. 2-ya Baumanskaya 5/1, Moscow, 105005, Russian Federation
^eShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russian Federation
^fInstitute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, Prosp. Entuziastov 13, Saratov, 410049, Russian Federation
^gSyntol Ltd., Timiryazevskaya str. 42, Bldg B, room 316, Moscow, 127434, Russian Federation

This article gives a brief overview of the state of the art in optical methods for the detection of single molecules in biomedical applications. It has been shown that recording the fluorescence of single dye molecules covalently bound to antibodies (biomolecules), together with the use of modern nanophotonics methods, can be used to solve various problems in biology and medicine: (1) visualization of biomolecules, toxins, virus particles, (2) determination of extremely low concentrations of analytes directly in a collected sample without using methods to increase the concentration of the analyte, (3) optical single-molecule sequencing of DNA molecules. The existing physical limitations of methods for optical detection and counting of single molecules and their implications for solving existing problems in biology, medicine and genetics will be discussed.

Keywords: nanophotonics, nanoplasmonics, detection of ultra-low concentrations of analytes, single molecules detection, biovisualization, zero mode waveguide, single-molecule-real-time sequencing
DOI: 10.3367/UFNe.2024.07.039720
Citation: Melentiev P N, Kalmykov A S, Gritchenko A S, Shemeteva M P, Safonova A M, Markov M S, Balykin V I, Bukatin A S, Vaulin N V, Belov D A, Evstrapov A A, Baklykov D A, Andriyash A V, Barbasheva A A, Kuguk A K, Ryzhkov V V, Rodionov I A, Kudryavtsev D S, Mozhaeva V A, Son L V, Tsetlin V I, Khlebtsov B N, Kobzev M S, Kuznetsova Yu O, Sharipov B T, Yashkin A S, Alekseev Ya I "Optical methods for the detection of single biomolecules: visualization, detection and sequencing of DNA molecules" Phys. Usp., accepted

Received: 13th, March 2024, revised: 16th, July 2024, accepted: 17th, July 2024

Оригинал: Мелентьев П Н, Калмыков А С, Гритченко А С, Шеметева М П, Сафонова А М, Марков М С, Балыкин В И, Букатин А С, Ваулин Н В, Белов Д А, Евстрапов А А, Баклыков Д А, Андрияш А В, Барбашева А А, Кугук А К, Рыжков В В, Родионов И А, Кудрявцев Д С, Можаева В А, Сон Л В, Цетлин В И, Хлебцов Б Н, Кобзев М С, Кузнецова Ю О, Шарипов Б Т, Яшкин А С, Алексеев Я И «Оптические методы детектирования единичных биомолекул: визуализация, сенсорика, секвенирование молекул ДНК» УФН, принята к публикации; DOI: 10.3367/UFNr.2024.07.039720

Accepted articles

Optical methods for the detection of single biomolecules: visualization, detection and sequencing of DNA molecules