The metabolic component оf refractivity is considered as an example of а quantifying parameter for the 'vitality' of cells and organelles. It is shown that intracellular processes can be studied by the dynamic phase microscopy method, which provides real-time information in the form of local changes in the optical path difference. Preliminary results demonstrate the possibility of localizing а 'signal' and obtaining information under the signal – structure – function scheme and show prospects for conducting а 'dialogue' with а cell by real-time registering the way in which it responds to changes in external conditions. The mechanism underlying the dependence of refractivity on metabolic states is ехрlained by the contribution of bound water molecules.

PACS: 07.60.−j, 87.16.−b, 87.64.−t (all) DOI: 10.1070/PU2007v050n05ABEH006222 URL: https://ufn.ru/en/articles/2007/5/c/ 000249374100003 2-s2.0-34548584059 2007PhyU...50..513T Citation: Tychinskii V P "Dynamic phase microscopy: is a "dialogue" with the cell possible?" Phys. Usp. 50 513–528 (2007) BibTexBibNote ® (generic)BibNote ® (RIS)MedlineRefWorks Оригинал: Тычинский В П «Динамическая фазовая микроскопия: возможен ли "диалог" с клеткой?» УФН 177 535–552 (2007); DOI: 10.3367/UFNr.0177.200705c.0535